【文獻(xiàn)標(biāo)題】LncRNA MEG3 inhibits the infammatory response of ankylosing spondylitis by targeting miR‑146a

【作者】Yehong Li，Shanshan Zhang，Cunxin Zhang，et.al

【作者單位】濟(jì)寧市第一人民醫(yī)院（Jining No.1 People’s Hospital）

【文獻(xiàn)中引用產(chǎn)品】

人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒

人白細(xì)胞介素-6（IL-6）ELISA試劑盒

人腫瘤壞死因子-α(TNF-α)ELISA試劑盒

【關(guān)鍵詞】Ankylosing spondylitis，MEG3，miR-146a，Infammatory response，Cytokines

【DOI】https://doi.org/10.1007/s11010-019-03681-x

【影響因子(IF)】5.10

【出版期刊】《Molecular and Cellular Biochemistry》

【產(chǎn)品原文引用】

Enzyme‑linked immunosorbent assay (ELISA)

The levels of infammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were detected by ELISA kit (Hengyuan, Shanghai, China). In short, the samples to be tested and the standard samples were added to the corresponding enzyme plate and cultured at 37 ℃ for 1 h. After washing with PBS, the chromogenic antibody was added to the enzyme plates and continued incubation for 30 min. Finally, the termination solution was added to terminate the reaction. OD value at 450 nm was measured by a microplate reader (Thermo Fisher Scientifc, Rockford, IL, USA).

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