恒遠產品文獻:豬MMP-9,MPO,LTF,calprotein ELISA試劑盒引用文獻
發(fā)布時間:2021/3/30 16:18:14 閱讀次數(shù)：871

【文獻標題】Exposure to the environmental pollutant ammonia causes changes in gut microbiota and inflammatory markers in fattening pigs

【作者】Yutao Li， Runxiang Zhang，Xiang Liet，et.al

【作者單位】東北農業(yè)大學（Northeast Agricultural University）

【文獻中引用產品】

豬基質金屬蛋白酶-9（MMP-9）ELISA試劑盒

豬髓過氧化物酶（MPO）ELISA試劑盒

豬乳鐵蛋白(LTF)ELISA試劑盒

豬鈣衛(wèi)蛋白（calprotein）ELISA試劑盒

【關鍵詞】Ammonia，L-Selenomethionine，Gut microbiota，Inflammatory markers，Livestocks

【DOI】https://doi.org/10.1016/j.ecoenv.2020.111564

【影響因子(IF)】6.2

【出版期刊】《Ecotoxicology and Environmental Safety 》

【產品原文引用】

2.2. Detection of inflammatory marker levels in feces The collected fecal samples were diluted with cold physiological saline at a ratio of 1:9 and then centrifuged at 3,500 rpm for 10 min at 4 ℃. The supernatant was used for the subsequent analysis of inflammatory marker levels. The contents of Matrix metalloproteinase-9 (MMP-9), Myeloperoxidase (MPO), Lactoferrin (LTF) and Calprotectin were detected by corresponding ELISA assay kits (Shanghai Hengyuan biological technology Co., Ltd., Shanghai, China). All procedure was followed by the instructions of the manufacturer. The absorbance values were determined by the spectrophotometer (SpectraMax? ABS00254, California, USA). The maximum coefficient of variation presented a good reproducibility. Then, OD values were measured by enzymelabeled instrument at 450 nm. The relative contents of all inflammatory markers were calculated based on corresponding formulas.